Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores

Tsoraev G, Protasova E, Klimanova E, Ryzhykau Y, Kuklin A, Semenov Y, Ge B, Li W, Qin S, Friedrich T, Sluchanko N, Maksimov E
Structural Dynamics 9(5):054701 (2022 Sep)

doi: 10.1063/4.0000164
Submitted to SASBDB: 2022 Jun 14
Published in SASBDB:

SASDPA4 – SAXS data for the trimeric C-phycocyanin

C-phycocyanin alpha subunitC-phycocyanin beta subunit experimental SAS data
GASBOR model
Sample: C-phycocyanin alpha subunit trimer, 53 kDa Arthrospira platensis protein
C-phycocyanin beta subunit trimer, 54 kDa Arthrospira platensis protein
Buffer: 150 mM NaCl, 20 mM Tris, pH: 7.6
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 154 nm3

SASDPB4 – SAXS data for the C-phycocyanin in 8 M urea solution

C-phycocyanin alpha subunitC-phycocyanin beta subunit experimental SAS data
GASBOR model
Sample: C-phycocyanin alpha subunit monomer, 18 kDa Arthrospira platensis protein
C-phycocyanin beta subunit monomer, 18 kDa Arthrospira platensis protein
Buffer: 8 M urea, 30 mM βME, pH: 7
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14
RgGuinier 6.6 nm
Dmax 23.5 nm