Structural analysis of the Candida albicans mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism.

Tarrés-Solé A, Battistini F, Gerhold JM, Piétrement O, Martínez-García B, Ruiz-López E, Lyonnais S, Bernadó P, Roca J, Orozco M, Le Cam E, Sedman J, Solà M
Nucleic Acids Res (2023 May 19)

PMID: 37207342 doi: 10.1093/nar/gkad397

Submitted to SASBDB: 2022 Jul 11 Published in SASBDB: 2023 Jun 2

SASDPY5 – Gcf1p protein Sample: Gcf1p monomer, 26 kDa Candida albicans (strain … protein Buffer: 50mM Tris, 750mM NaCl, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 Oct 10 RgGuinier 3.7 nm Dmax 14.5 nm VolumePorod 80 nm3

SASDPZ5 – Gcf1p protein bound to DNA at 1.0 mg/mL Sample: Gcf1p monomer, 26 kDa Candida albicans (strain … protein Af2_20 DNA monomer, 12 kDa DNA Buffer: 25 mM Tris, 20 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30 RgGuinier 3.6 nm Dmax 16.0 nm VolumePorod 92 nm3

SASDP26 – Gcf1p protein bound to DNA at 2.0 mg/mL Sample: Gcf1p monomer, 26 kDa Candida albicans (strain … protein Af2_20 DNA monomer, 12 kDa DNA Buffer: 25 mM Tris, 20 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30 RgGuinier 3.9 nm Dmax 16.0 nm VolumePorod 133 nm3

SASDP36 – N-terminal truncated Gcf1p (amino acids 59-245) bound to DNA Sample: Af2_20 DNA monomer, 12 kDa DNA Gcf1p(Δ58) monomer, 22 kDa Candida albicans (strain … protein Buffer: 25 mM Tris, 20 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30 RgGuinier 2.6 nm Dmax 10.5 nm VolumePorod 52 nm3