Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2.

Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
Commun Biol 5(1):925 (2022 Sep 7)

PMID: 36071105 doi: 10.1038/s42003-022-03892-x

Submitted to SASBDB: 2022 Jul 19 Published in SASBDB: 2022 Oct 18

SASDP56 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 250 mM NaCl buffer Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4 Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19 RgGuinier 4.1 nm

SASDP66 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 500 mM NaCl buffer Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4 Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 98 nm3

SASDP76 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 1000 mM NaCl buffer Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4 Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 100 nm3

SASDP86 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 250 mM NaCl buffer Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4 Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19 RgGuinier 6.3 nm Dmax 24.0 nm

SASDP96 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 500 mM NaCl buffer Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4 Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19 RgGuinier 5.2 nm Dmax 18.0 nm

SASDPA6 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4 Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19 RgGuinier 4.8 nm Dmax 16.0 nm