Structural plasticity of NFU1 upon interaction with binding partners: insights into the mitochondrial [4Fe-4S] cluster pathway

Da Vela S, Saudino G, Lucarelli F, Banci L, Svergun D, Ciofi-Baffoni S
Journal of Molecular Biology :168154 (2023 May)

doi: 10.1016/j.jmb.2023.168154 		Submitted to SASBDB: 2022 Aug 17
Published in SASBDB:

SASDP98 – Human apo iron-sulfur cluster assembly 1 (ISCA1)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
DAMMIF model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
RgGuinier 1.9 nm
Dmax 6.8 nm
VolumePorod 25 nm3

SASDPA8 – Human apo NFU1 iron-sulfur cluster scaffold protein

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) experimental SAS data
Human apo NFU1 iron-sulfur cluster scaffold protein Rg histogram
Sample: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) monomer, 22 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
RgGuinier 2.4 nm
Dmax 9.3 nm
VolumePorod 32 nm3

SASDPB8 – Human apo iron-sulfur cluster assembly 1/NFU1 iron-sulfur cluster scaffold protein complex (ISCA1-NFU1)

Iron-sulfur cluster assembly 1 homolog, mitochondrialNFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) experimental SAS data
DAMMIF model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) monomer, 22 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 43 nm3

SASDPC8 – Human apo iron-sulfur cluster assembly 1/iron-sulfur cluster assembly 2 complex (ISCA1-ISCA2)

Iron-sulfur cluster assembly 1 homolog, mitochondrialIron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
DAMMIF model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 43 nm3

SASDPD8 – Human apo iron-sulfur cluster assembly 1/iron-sulfur cluster assembly 2/NFU1 iron-sulfur cluster scaffold protein complex (ISCA1-ISCA2-NFU1)

Iron-sulfur cluster assembly 1 homolog, mitochondrialNFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G)Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
CORAL model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) monomer, 22 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 27
RgGuinier 3.2 nm
Dmax 11.7 nm
VolumePorod 70 nm3