Antiviral signalling by a cyclic nucleotide activated CRISPR protease.

Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
Nature 614(7946):168-174 (2023 Feb)

PMID: 36423657 doi: 10.1038/s41586-022-05571-7

Submitted to SASBDB: 2022 Sep 6 Published in SASBDB: 2022 Nov 10

SASDQM4 – CRISPR associated Lon protease (CalpL) Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14 RgGuinier 3.1 nm Dmax 10.3 nm VolumePorod 80 nm3

SASDQN4 – Complex of CRISPR associated Lon protease (CalpL) with a 10 kDa C-terminal fragment of CalpT a putative MazF-like toxin (CalpT10) Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein Uncharacterized protein (putative MazF-like toxin) monomer, 9 kDa Sulfurihydrogenibium sp. (strain … protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14 RgGuinier 3.4 nm Dmax 11.7 nm VolumePorod 93 nm3

SASDQP4 – CRISPR associated Lon protease (CalpL) in the presence of cyclic oligoadenylate cA4 Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8 RgGuinier 3.2 nm Dmax 10.3 nm VolumePorod 82 nm3

SASDQQ4 – CRISPR associated Lon protease (CalpL) asymmetrically dimerizing in the presence of cyclic oligoadenylate cA4 Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8 RgGuinier 3.8 nm Dmax 13.0 nm VolumePorod 116 nm3