Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein.

Rawlinson SM, Chakraborty S, Sethi A, David CT, Harrison AR, Bird LE, Rozario AM, Uthishtran S, Ardipradja K, Zhao T, Oksayan S, Jans DA, Ang CS, Lu ZH, Yan F, Williamson NA, Arumugam S, Sundaramoorthy V, Bell TDM, Gooley PR, Moseley GW
Nat Commun 16(1):9491 (2025 Oct 29)

PMID: 41193489 doi: 10.1038/s41467-025-65223-y

Submitted to SASBDB: 2023 Jan 29 Published in SASBDB: 2025 Nov 6

SASDRB6 – Rabies CVS Phosphoprotein (P1) Sample: Phosphoprotein dimer, 67 kDa Rabies virus (strain … protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7 RgGuinier 4.7 nm Dmax 25.0 nm VolumePorod 230 nm3

SASDRX4 – Rabies virus CVS Phosphoprotein Isoform 3 (CVS P3) Sample: Phosphoprotein (Isoform P3; C297S) dimer, 56 kDa Rabies virus (strain … protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7 RgGuinier 4.2 nm Dmax 17.0 nm VolumePorod 141 nm3

SASDRZ4 – K214/R260 mutant of Rabies virus CVS Phosphoprotein Isoform 3 (KRm CVS P3) Sample: Phosphoprotein (Isoform P3; K214A, R260A, D289N, C297S) dimer, 55 kDa Rabies virus (strain … protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7 RgGuinier 4.6 nm Dmax 22.5 nm VolumePorod 180 nm3

SASDRY4 – D289N mutant of Rabies virus CVS Phosphoprotein Isoform 3 (CVS P3) Sample: Phosphoprotein (Isoform P3; D289N, C297S) dimer, 56 kDa Rabies virus (strain … protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7 RgGuinier 3.9 nm Dmax 17.0 nm VolumePorod 128 nm3