Structure(s) of Monomeric State of Alpha Synuclein in Solution Solved by Combining SAXS and Alphafold

Submitted to SASBDB: 2022 Oct 31
Published in SASBDB:

SASDQJ7 – Alpha Synuclein at 283 K and concentration of 8 mg/ml

Human alpha-synuclein experimental SAS data
ALPHAFOLD model
Sample: Human alpha-synuclein monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2015 Mar 18
RgGuinier 4.7 nm
Dmax 17.6 nm
VolumePorod 81 nm3

SASDQK7 – Alpha Synuclein at 283 K and concentration of 3.8 mg/ml

Human alpha-synuclein experimental SAS data
Human alpha-synuclein Kratky plot
Sample: Human alpha-synuclein monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2015 Mar 18
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 76 nm3