Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer

Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
Angewandte Chemie International Edition (2023 Nov 04)

doi: 10.1002/anie.202312517

Submitted to SASBDB: 2023 Jan 9 Published in SASBDB: 2023 Nov 3

SASDQ97 – Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Buffer: 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 7 RgGuinier 3.0 nm Dmax 14.2 nm VolumePorod 61 nm3

SASDQT9 – Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with fourth BRC repeat (BRC4) Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein monomer, 4 kDa Homo sapiens protein Buffer: 20 mM HEPES, 50 mM Na2SO4, 2% v/v sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Dec 7 RgGuinier 2.9 nm Dmax 15.6 nm VolumePorod 70 nm3

SASDQU9 – His-Tagged Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with His-tagged fourth BRC repeat (BRC4) Sample: DNA repair protein RAD51 homolog 1 (F86E, A89E, His-Tagged) monomer, 40 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein monomer, 6 kDa Homo sapiens protein Buffer: 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 7 RgGuinier 3.0 nm Dmax 16.5 nm VolumePorod 78 nm3