The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.

Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
Nucleic Acids Res (2024 Jan 9)

PMID: 38197228 doi: 10.1093/nar/gkad1241

Submitted to SASBDB: 2023 Apr 13 Published in SASBDB: 2024 Jan 13

SASDRX8 – Heterodimeric complex of AfAgo protein Af1318 and AfAgo-N protein (containing N-L1-L2 domains) with a 14 base pair DNA oligoduplex in low salt buffer Sample: Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein) monomer, 51 kDa Archaeoglobus fulgidus (strain … protein Uncharacterized protein (AfAgo-N protein containing N-L1-L2 domains) monomer, 31 kDa Archaeoglobus fulgidus DSM … protein 5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA Buffer: 20 mM TrisHCl pH 7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6 RgGuinier 2.9 nm Dmax 9.7 nm VolumePorod 145 nm3

SASDR29 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant in high salt buffer Sample: Single-chain full Archaeoglobus fulgidus Argonaute variant monomer, 78 kDa Archaeoglobus fulgidus protein Buffer: 20 mM TrisHCl pH 7.5, 500 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31 RgGuinier 3.1 nm Dmax 9.7 nm VolumePorod 142 nm3

SASDRZ8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with an 11 base pair DNA oligoduplex in low salt buffer Sample: Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein 5'-end phosphorylated DNA guide strand, 11 nt (MZ864) monomer, 3 kDa DNA DNA target strand, 11 nt (MZ865) monomer, 3 kDa DNA Buffer: 20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31 RgGuinier 2.9 nm Dmax 9.6 nm VolumePorod 125 nm3

SASDRY8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with a 14 base pair DNA oligoduplex in low salt buffer Sample: Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein 5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA Buffer: 20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6 RgGuinier 3.0 nm Dmax 10.3 nm VolumePorod 131 nm3