Compact polyethylenimine-complexed mRNA vaccines

Moreno Herrero J, Stahl T, Erbar S, Maxeiner K, Schlegel A, Bacic T, Schumacher J, Cavalcanti L, Schroer M, Svergun D, Sahin U, Haas H
Nature Nanotechnology (2025 Aug 11)

doi: 10.1038/s41565-025-01961-w 		Submitted to SASBDB: 2023 Apr 25
Published in SASBDB:

SASDRK9 – single self-amplifying RNA

self-amplifying RNA experimental SAS data
DAMMIF model
Sample: Self-amplifying RNA monomer, 3030 kDa RNA
Buffer: MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 10
RgGuinier 90.5 nm
Dmax 200.0 nm

SASDRL9 – single self-amplifying RNA + 50 mM NaCl

self-amplifying RNA experimental SAS data
DAMMIF model
Sample: Self-amplifying RNA monomer, 3030 kDa RNA
Buffer: MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 10
RgGuinier 30.7 nm
Dmax 107.4 nm
VolumePorod 112000 nm3

SASDRM9 – Polyethylenimine-compacted single self-amplifying RNA for prophylactic and therapeutic application, specifically for vaccination

self-amplifying RNAlinear polyethylenimine experimental SAS data
DAMMIF model
Sample: Self-amplifying RNA monomer, 3030 kDa RNA
Linear polyethylenimine monomer, 25 kDa none (polymer)
Buffer: MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 10
RgGuinier 12.0 nm
Dmax 40.0 nm
VolumePorod 9100 nm3