The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces.

Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N
J Biol Chem :105356 (2023 Oct 18)

PMID: 37863265 doi: 10.1016/j.jbc.2023.105356

Submitted to SASBDB: 2023 Aug 9 Published in SASBDB: 2023 Sep 5

SASDSE8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.27 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 2.7 nm Dmax 7.1 nm VolumePorod 67 nm3

SASDSF8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.55 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 2.8 nm Dmax 8.0 nm VolumePorod 65 nm3

SASDSG8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.07 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 2.9 nm Dmax 9.0 nm VolumePorod 70 nm3

SASDSH8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.88 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 3.0 nm Dmax 9.7 nm VolumePorod 74 nm3

SASDSJ8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 4.37 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 3.0 nm Dmax 9.9 nm VolumePorod 76 nm3

SASDSK8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 6.38 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 3.0 nm Dmax 9.9 nm VolumePorod 77 nm3

SASDSL8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 7.51 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 2.9 nm Dmax 9.6 nm VolumePorod 77 nm3

SASDSM8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 9.15 mg/mL Sample: Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21 RgGuinier 2.9 nm Dmax 10.8 nm VolumePorod 78 nm3