Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA

Stephanie Hicks

Submitted to SASBDB: 2023 May 18 Published in SASBDB: 2025 May 18

SASDSV2 – spikemoss tandem zinc finger (TZF) domain, pH 8 at 5.5 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.8 nm Dmax 7.3 nm VolumePorod 15 nm3

SASDSW2 – spikemoss tandem zinc finger (TZF) domain, pH 8 at 4.1 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.7 nm Dmax 7.1 nm VolumePorod 14 nm3

SASDSX2 – spikemoss tandem zinc finger (TZF) domain, pH 8 at 2.75 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.7 nm Dmax 7.1 nm VolumePorod 14 nm3

SASDSY2 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 17.1 mg/mL Sample: Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 19 RgGuinier 1.0 nm Dmax 3.5 nm VolumePorod 5 nm3

SASDSZ2 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 17.1 mg/mL (repeat) Sample: Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 19 RgGuinier 1.0 nm Dmax 3.5 nm VolumePorod 5 nm3

SASDS23 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 14 mg/mL Sample: Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 19 RgGuinier 1.0 nm Dmax 3.2 nm VolumePorod 5 nm3

SASDS33 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 5 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.6 nm Dmax 5.6 nm VolumePorod 13 nm3

SASDS43 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 3 mg/mL Sample: Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.5 nm Dmax 5.7 nm VolumePorod 13 nm3

SASDS53 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 2 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.5 nm Dmax 5.6 nm VolumePorod 13 nm3

SASDS63 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 6 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.5 nm Dmax 5.1 nm VolumePorod 13 nm3

SASDS73 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 4.5 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.5 nm Dmax 4.9 nm VolumePorod 13 nm3

SASDS83 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 3 mg/mL Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9 RgGuinier 1.5 nm Dmax 5.3 nm VolumePorod 13 nm3