Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex

Tomas Brom

Submitted to SASBDB: 2023 Jul 13 Published in SASBDB: 2024 Jun 13

SASDSE7 – Human CDAN1-interacting nuclease 1 (CDIN1): analysis of SEC peak Sample: CDAN1-interacting nuclease 1 dimer, 67 kDa Homo sapiens protein Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Sep 2 RgGuinier 3.0 nm Dmax 8.2 nm VolumePorod 84 nm3

SASDSF7 – Human Codanin1 (C-terminus): analysis of second monomeric SEC peak Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5 RgGuinier 2.7 nm Dmax 7.5 nm VolumePorod 54 nm3

SASDSG7 – Human Codanin1 (C-terminus): analysis of first oligomeric SEC peak Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5 RgGuinier 4.1 nm Dmax 10.7 nm VolumePorod 220 nm3