Recombinant expression and purification of Plasmodium heme detoxification protein in E. coli: Challenges and discoveries.

Singh R, Makde RD
Protein Expr Purif 236:106789 (2025 Aug 7)
PMID: 40759288
doi: 10.1016/j.pep.2025.106789 		Submitted to SASBDB: 2025 Jun 12
Published in SASBDB:

SASDXF5 – Chaperone protein DnaK (E.coli) & recombinant Heme ligase (HDP) (P.fulciparum) protein complex

Chaperone protein DnaKHeme ligase experimental SAS data
GASBOR model
Sample: Chaperone protein DnaK, 69 kDa Escherichia coli (strain … protein
Heme ligase, 28 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-Cl, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, Chemistry Division, Bhabha Atomic Research Centre on 2019 Feb 26
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 83 nm3