Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.

Dao TP, Rajendran A, Galagedera SKK, Haws W, Castañeda CA
Biophys J (2023 Nov 30)

PMID: 38041404 doi: 10.1016/j.bpj.2023.11.3401

Submitted to SASBDB: 2023 Jun 21 Published in SASBDB: 2023 Sep 27

SASDS96 – Human Ubiquilin-1 (UBQLN1) Sample: Ubiquilin-1 dimer, 125 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22 RgGuinier 4.6 nm Dmax 19.3 nm VolumePorod 264 nm3

SASDSA6 – Human Ubiquilin-2 (UBQLN2) Sample: Ubiquilin-2 dimer, 131 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8 RgGuinier 5.2 nm Dmax 22.5 nm VolumePorod 330 nm3

SASDSB6 – Human Ubiquilin-4 (UBQLN4) Sample: Ubiquilin-4 dimer, 128 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26 RgGuinier 5.1 nm Dmax 21.7 nm VolumePorod 333 nm3