UBQLN2 domain deletion constructs

Sarasi Galagedera

Submitted to SASBDB: 2023 Jun 21 Published in SASBDB: 2025 Jun 21

SASDSC6 – Human Ubiquilin-2, ∆STI1-1 domain deletion (UBQLN2∆STI1-1) Sample: Ubiquilin-2 (∆170-260) dimer, 110 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16 RgGuinier 5.4 nm Dmax 26.0 nm VolumePorod 357 nm3

SASDSD6 – Human Ubiquilin-2 with ∆UBL and ∆STI1-1 domain deletions (UBQLN2∆STI1-1∆UBL) Sample: Ubiquilin-2 (∆33-103, ∆170-260) dimer, 94 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16 RgGuinier 5.6 nm Dmax 24.5 nm VolumePorod 326 nm3

SASDSE6 – Human Ubiquilin-2, ∆1-108 N-terminal deletion (UBQLN2 109-624) Sample: Ubiquilin-2 (∆1-108) dimer, 108 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16 RgGuinier 5.2 nm Dmax 23.3 nm VolumePorod 281 nm3

SASDSF6 – Human Ubiquilin-2, C-terminal construct (UBQLN2 450-624W) Sample: Ubiquilin-2 (∆1-449)-W dimer, 35 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16 RgGuinier 3.8 nm Dmax 18.5 nm VolumePorod 50 nm3

SASDSJ6 – Human Ubiquilin-2, P497E phosphomimetic point mutant (UBQLN2 P497E) Sample: Ubiquilin-2 (P497E) dimer, 131 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8 RgGuinier 5.3 nm Dmax 21.3 nm VolumePorod 340 nm3

SASDSK6 – Human Ubiquilin-2, P497L ALS-linked point mutant (UBQLN2 P497L) Sample: Ubiquilin-2 (P497L) dimer, 131 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8 RgGuinier 7.3 nm Dmax 28.8 nm VolumePorod 1365 nm3

SASDSL6 – Human Ubiquilin-2, R12G R15D Q21E triple point mutant (UBQLN2 GDE) Sample: Ubiquilin-2 (R12G, R15D, Q21E) dimer, 131 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22 RgGuinier 5.1 nm Dmax 21.3 nm VolumePorod 330 nm3

SASDSM6 – Human Ubiquilin-2, ∆551-569 deletion construct (UBQLN2∆551-569) Sample: Ubiquilin-2 (∆551-569) dimer, 127 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22 RgGuinier 5.2 nm Dmax 23.4 nm VolumePorod 332 nm3