Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP

Raktim Roy

Submitted to SASBDB: 2023 Aug 2 Published in SASBDB: 2025 Aug 2

SASDSR7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form without any substrates Sample: Gramicidin S synthase 1 (W239S) monomer, 127 kDa Aneurinibacillus migulanus protein Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14 RgGuinier 4.1 nm Dmax 15.0 nm VolumePorod 187 nm3

SASDSS7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14 RgGuinier 4.3 nm Dmax 15.4 nm VolumePorod 186 nm3

SASDST7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP) Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14 RgGuinier 4.2 nm Dmax 16.0 nm VolumePorod 186 nm3

SASDSU7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14 RgGuinier 4.1 nm Dmax 17.5 nm VolumePorod 181 nm3

SASDSV7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP) Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14 RgGuinier 4.0 nm Dmax 14.3 nm VolumePorod 175 nm3