SAXS studies of KAI2 catalytic mutants

Sabrina Davies

Submitted to SASBDB: 2023 Aug 7 Published in SASBDB: 2025 May 15

SASDSY7 – Karrikin insensitive 2, KAI2 protein (monomer peak) Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 2.3 nm Dmax 9.5 nm VolumePorod 58 nm3

SASDSZ7 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak) Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 2.9 nm Dmax 13.9 nm VolumePorod 60 nm3

SASDS28 – Karrikin insensitive 2, KAI2 protein (aggregate peak) Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 4.1 nm Dmax 15.8 nm VolumePorod 283 nm3

SASDS38 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak) Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 4.9 nm Dmax 20.0 nm VolumePorod 345 nm3

SASDS48 – Karrikin insensitive 2, KAI2 protein mutant S95A (monomer peak) Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 1.9 nm Dmax 6.3 nm VolumePorod 55 nm3

SASDS58 – Karrikin insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (monomer peak) Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 2.1 nm Dmax 7.2 nm VolumePorod 61 nm3

SASDS68 – Karrikin insensitive 2, KAI2 protein mutant S95A (aggregate peak) Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 3.9 nm Dmax 13.2 nm VolumePorod 171 nm3

SASDS78 – Karrikin insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (aggregate peak) Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 4.5 nm Dmax 19.3 nm VolumePorod 285 nm3

SASDS88 – Karrikin insensitive 2, KAI2 protein mutant S95C (monomer peak) Sample: Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 1.9 nm Dmax 5.7 nm VolumePorod 45 nm3

SASDS98 – Karrikin insensitive 2, KAI2 protein mutant S95C with a synthetic strigolactone analogue ligand (monomer peak) Sample: Probable esterase KAI2 - S95C (KARRIKIN INSENSITIVE 2 - S95C) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 1.9 nm Dmax 5.7 nm VolumePorod 35 nm3

SASDSA8 – Karrikin insensitive 2, KAI2 protein mutant H246Y (monomer peak) Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 3.0 nm Dmax 16.1 nm VolumePorod 70 nm3

SASDSB8 – Karrikin insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (monomer peak) Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 2.9 nm Dmax 13.2 nm VolumePorod 68 nm3

SASDSC8 – Karrikin insensitive 2, KAI2 protein mutant H246Y (aggregate peak) Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 5.6 nm Dmax 23.0 nm VolumePorod 494 nm3

SASDSD8 – Karrikin insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (aggregate peak) Sample: Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29 RgGuinier 5.1 nm Dmax 19.3 nm VolumePorod 403 nm3