Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering

Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
International Journal of Molecular Sciences 24(17):13649 (2023 Sep 04)

doi: 10.3390/ijms241713649

Submitted to SASBDB: 2023 Aug 30 Published in SASBDB: 2023 Sep 1

SASDS69 – Full length SARS-CoV-2 Non-structural protein 10 (Nsp10) Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25 RgGuinier 1.7 nm Dmax 5.9 nm VolumePorod 21 nm3

SASDS79 – Long SARS-CoV-2 Non-structural protein 10 (Nsp10: C-terminal truncation variant) Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25 RgGuinier 1.6 nm Dmax 5.4 nm VolumePorod 19 nm3

SASDS89 – Short SARS-CoV-2 Non-structural protein 10 (Nsp10: N- and C-terminal truncation variant) Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25 RgGuinier 1.5 nm Dmax 5.1 nm VolumePorod 16 nm3

SASDS99 – Full length SARS Non-structural protein 10 (Nsp10) Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6 RgGuinier 1.7 nm Dmax 5.9 nm VolumePorod 19 nm3

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10) Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6 RgGuinier 1.7 nm Dmax 5.6 nm VolumePorod 19 nm3