The NS1 protein of influenza B virus binds 5’-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response

Woltz R, Schweibenz B, Tsutakawa S, Zhao C, Ma L, Shurina B, Hura G, John R, Vorobiev S, Swapna G, Solotchi M, Tainer J, Krug R, Patel S, Montelione G
(2024 Jan 24)

doi: 10.1101/2023.09.25.559316

Submitted to SASBDB: 2023 Nov 23 Published in SASBDB: 2025 Nov 23

SASDT55 – Non-structural protein 1 (NS1B, C-terminal) with 5' PPP hairpin RNA Sample: Non-structural protein 1 monomer, 16 kDa Influenza B virus protein 5’ppp ds10 HP RNA monomer, 8 kDa RNA Buffer: 40 mM ammonium acetate, pH 5.5, 225 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6 RgGuinier 2.0 nm Dmax 6.9 nm VolumePorod 28 nm3

SASDT65 – Non-structural protein 1 (NS1B, C-terminal) plus double stranded RNA Sample: Non-structural protein 1 monomer, 16 kDa Influenza B virus protein RNA top strand monomer, 5 kDa RNA RNA bottom strand monomer, 5 kDa RNA Buffer: 40 mM ammonium acetate, pH 5.5, 450 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 18 RgGuinier 2.8 nm Dmax 10.3 nm VolumePorod 23 nm3