Solution structure and functional characterization of an avian picornavirus 2AH/NC protein

Anna Wehlin Submitted to SASBDB: 2023 Sep 26
Published in SASBDB:

SASDTR2 – Turkey Avisivirus 2A3 (TuAsV 2A3) polyprotein fragment at pH 5.4

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM citrate pH 5.4, 150 mM NaCl, 2 mM TCEP, 1% glycerol, pH: 5.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
RgGuinier 1.7 nm
Dmax 7.2 nm
VolumePorod 29 nm3

SASDTS2 – Turkey Avisivirus 2A3 (TuAsV 2A3) polyprotein fragment at pH 6.5

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20mM Citrate buffer pH 6.5, 150mM NaCl, 2mM TCEP, 1% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 May 15
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 27 nm3

SASDTT2 – Turkey Avisivirus 2A3 (TuAsV 2A3) polyprotein fragment at pH 7.4

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TECP, 1% Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
RgGuinier 1.6 nm
Dmax 4.0 nm
VolumePorod 24 nm3