Preorganized cyclic modules facilitate the self-assembly of protein nanostructures

Snoj J, Lapenta F, Jerala R
Chemical Science (2024)

doi: 10.1039/D3SC06658D

Submitted to SASBDB: 2024 Feb 1 Published in SASBDB: 2024 Feb 2

SASDUN2 – DiTET-cc (coiled coil protein origami tetrahedron assembled from two cyclized chains) Sample: CyRH1 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein CyRH2 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 30 RgGuinier 3.1 nm Dmax 8.8 nm VolumePorod 150 nm3

SASDUP2 – DiTET-nc (coiled coil protein origami tetrahedron assembled from one linear and one cyclized chain) Sample: RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein CyRH2 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein Buffer: 50 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Aug 29 RgGuinier 3.6 nm Dmax 12.5 nm VolumePorod 161 nm3

SASDUQ2 – DiTET-nn (coiled coil protein origami assembled from two linear chains) Sample: RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein RH2 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 9 RgGuinier 4.3 nm Dmax 14.7 nm VolumePorod 161 nm3

SASDUR2 – SB24-nnn (coiled coil protein origami irregular octahedron assembled from three linear chains) Sample: SB6 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein SB9b (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 42 kDa synthetic construct protein SB9c (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 41 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 25 RgGuinier 4.8 nm Dmax 16.1 nm VolumePorod 443 nm3

SASDUS2 – SB24-ncn (coiled coil protein origami irregular octahedron assembled from 2 linear chains and 1 cyclic chain) Sample: SB9b (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 42 kDa synthetic construct protein SB9c (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 41 kDa synthetic construct protein CySB6 (Cyclic coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 9 RgGuinier 4.7 nm Dmax 14.7 nm VolumePorod 428 nm3