Molecular basis of the regulation of full-length Nedd4-2 through calcium and 14-3-3 proteins

Veronika Obšilová

Submitted to SASBDB: 2024 Feb 20 Published in SASBDB: 2026 Feb 20

SASDUB3 – 14-3-3 eta human Sample: 14-3-3 protein eta dimer, 57 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Dec 4 RgGuinier 2.9 nm Dmax 8.4 nm VolumePorod 85 nm3

SASDUE3 – Phosphorylated E3 ubiquitin-protein ligase Nedd4-2 complexed to 14-3-3 eta in the absence of calcium Sample: 14-3-3 protein eta dimer, 57 kDa Homo sapiens protein Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like monomer, 110 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17 RgGuinier 5.4 nm Dmax 21.4 nm VolumePorod 281 nm3

SASDUF3 – Phosphorylated E3 ubiquitin-protein ligase Nedd4-2 complexed to 14-3-3 eta in the presence of calcium Sample: 14-3-3 protein eta dimer, 57 kDa Homo sapiens protein Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like monomer, 110 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 1 mM CaCl2, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Dec 4 RgGuinier 5.5 nm Dmax 23.5 nm VolumePorod 290 nm3