A Simple Protocol for Visualization of RNA-Protein Complexes by Atomic Force Microscopy.
Tripepi A,
Shakoor H,
Klapetek P
Curr Protoc
5(1):e70084
(2025 Jan)
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Sample: |
Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-177) dimer, 89 kDa Homo sapiens protein
3'UTR fragment of ADP-ribosylation factor 1 monomer, 14 kDa Homo sapiens RNA
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Buffer: |
50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH: 7 |
Experiment: |
SAXS
data collected at Rigaku BioSAXS-1000, CEITEC on 2020 May 4
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RgGuinier |
4.9 |
nm |
Dmax |
13.4 |
nm |
VolumePorod |
129 |
nm3 |
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Sample: |
3'UTR fragment of ADP-ribosylation factor 1 monomer, 14 kDa Homo sapiens RNA
Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated Staufen-swapping (ΔSSM) dimer, 81 kDa Homo sapiens protein
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Buffer: |
50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH: 7 |
Experiment: |
SAXS
data collected at Rigaku BioSAXS-2000, CEITEC on 2024 Jan 12
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RgGuinier |
5.5 |
nm |
Dmax |
16.1 |
nm |
VolumePorod |
139 |
nm3 |
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