Structural Basis for Membrane Targeting and Secretion of Legionella SidE Ubiquitin Ligases

Ahmed Mohammed

Submitted to SASBDB: 2024 Mar 29 Published in SASBDB: 2025 Nov 19

SASDUW5 – C-terminal domain of Ubiquitinating/deubiquitinating enzyme SdeA (SdeA_CTD) Sample: Ubiquitinating/deubiquitinating enzyme SdeA monomer, 39 kDa Legionella pneumophila subsp. … protein Buffer: 25 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 4 RgGuinier 3.9 nm Dmax 15.7 nm VolumePorod 62 nm3

SASDUX5 – Type 4 adaptor protein complex IcmS, IcmW and DotL (IcmS/IcmW/DotLc) Sample: Type 4 adapter protein IcmS monomer, 14 kDa Legionella pneumophila subsp. … protein Type 4 adapter protein IcmW (S9A) monomer, 17 kDa Legionella pneumophila subsp. … protein Type 4 coupling protein DotL monomer, 15 kDa Legionella pneumophila subsp. … protein Buffer: 25 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 4 RgGuinier 2.8 nm Dmax 9.6 nm VolumePorod 71 nm3

SASDUY5 – C-terminal domain of Ubiquitinating/deubiquitinating enzyme SdeA bound to Type 4 adaptor protein complex IcmS, IcmW and DotL (SdeACTD-IcmSWDotLc complex) Sample: Ubiquitinating/deubiquitinating enzyme SdeA monomer, 39 kDa Legionella pneumophila subsp. … protein Type 4 adapter protein IcmS monomer, 14 kDa Legionella pneumophila subsp. … protein Type 4 adapter protein IcmW (S9A) monomer, 17 kDa Legionella pneumophila subsp. … protein Type 4 coupling protein DotL monomer, 15 kDa Legionella pneumophila subsp. … protein Buffer: 25 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 4 RgGuinier 3.8 nm Dmax 14.4 nm VolumePorod 166 nm3