Deciphering the allosteric regulation of mycobacterial inosine-5′-monophosphate dehydrogenase

Bulvas O, Knejzlík Z, Sýs J, Filimoněnko A, Čížková M, Clarová K, Rejman D, Kouba T, Pichová I
Nature Communications 15(1) (2024 Aug 06)

doi: 10.1038/s41467-024-50933-6 		Submitted to SASBDB: 2024 Mar 29
Published in SASBDB:

SASDUM5 – Mycobacterium smegmatis Inosine-5'-monophosphate dehydrogenase (IMPDH) - apo form

Inosine-5'-monophosphate dehydrogenase experimental SAS data
PYMOL model
Sample: Inosine-5'-monophosphate dehydrogenase octamer, 426 kDa Mycolicibacterium smegmatis (strain … protein
Buffer: 50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2024 Feb 21
RgGuinier 5.3 nm
Dmax 24.5 nm
VolumePorod 952 nm3

SASDUN5 – Mycobacterium smegmatis Inosine-5'-monophosphate dehydrogenase (IMPDH) - IMP-bound form

Inosine-5'-monophosphate dehydrogenase experimental SAS data
PYMOL model
Sample: Inosine-5'-monophosphate dehydrogenase tetramer, 213 kDa Mycolicibacterium smegmatis (strain … protein
Buffer: 50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2024 Mar 6
RgGuinier 5.0 nm
Dmax 21.5 nm
VolumePorod 471 nm3

SASDUP5 – Mycobacterium smegmatis Inosine-5'-monophosphate dehydrogenase (IMPDH) - ATP-bound form

Inosine-5'-monophosphate dehydrogenase experimental SAS data
PYMOL model
Sample: Inosine-5'-monophosphate dehydrogenase octamer, 426 kDa Mycolicibacterium smegmatis (strain … protein
Buffer: 50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2024 Jan 29
RgGuinier 5.0 nm
Dmax 14.8 nm
VolumePorod 821 nm3

SASDUQ5 – Mycobacterium smegmatis Inosine-5'-monophosphate dehydrogenase (IMPDH) - ATP+IMP-bound form

Inosine-5'-monophosphate dehydrogenase experimental SAS data
PYMOL model
Sample: Inosine-5'-monophosphate dehydrogenase octamer, 426 kDa Mycolicibacterium smegmatis (strain … protein
Buffer: 50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2024 Mar 6
RgGuinier 5.1 nm
Dmax 13.2 nm
VolumePorod 784 nm3

SASDUR5 – Mycobacterium smegmatis Inosine-5'-monophosphate dehydrogenase (IMPDH) - GTP-bound form

Inosine-5'-monophosphate dehydrogenase experimental SAS data
PYMOL model
Sample: Inosine-5'-monophosphate dehydrogenase octamer, 426 kDa Mycolicibacterium smegmatis (strain … protein
Buffer: 50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2024 Mar 6
RgGuinier 5.0 nm
Dmax 14.4 nm
VolumePorod 810 nm3