Insights into the stereochemical mechanism of the B(12)-dependent radical SAM glutamine C- methyltransferase (QCMT).

Bourdin T, Guillot A, Mauger M, Lefranc B, Gervason S, Glousieau M, Grimaldi S, Leprince J, Thureau A, Benjdia A, Berteau O
Commun Biol (2026 May 21)

PMID: 42168528 doi: 10.1038/s42003-026-10234-8

Submitted to SASBDB: 2024 May 17 Published in SASBDB: 2026 May 17

SASDVY7 – Glutamine C‐methyltransferase without cobalamin from Methanoculleus thermophilus Sample: B12-binding domain/radical SAM domain protein, MJ_0865 family monomer, 50 kDa Methanoculleus thermophilus protein Buffer: 50 mM Tris, 300 mM NaCl, 5% glycerol, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 2.6 nm Dmax 10.4 nm VolumePorod 85 nm3

SASDVZ7 – Glutamine C‐methyltransferase with cobalamin from Methanoculleus thermophilus Sample: B12-binding domain/radical SAM domain protein, MJ_0865 family monomer, 50 kDa Methanoculleus thermophilus protein Buffer: 50 mM Tris, 300 mM NaCl, 5% glycerol, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Sep 18 RgGuinier 2.5 nm Dmax 10.1 nm VolumePorod 78 nm3