The structure of the apolipoprotein A-I monomer provides insights into its oligomerisation and lipid-binding mechanisms

Tou H, Rosenes Z, Khandokar Y, Zlatic C, Metcalfe R, Mok Y, Morton C, Gooley P, Griffin M
Journal of Molecular Biology :169394 (2025 Aug)

doi: 10.1016/j.jmb.2025.169394

Submitted to SASBDB: 2024 May 31 Published in SASBDB: 2025 Sep 5

SASDU89 – Apolipoprotein A-I monomer, C-terminally truncated Sample: Apolipoprotein A-I monomer, 22 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 0.1% sodium azide, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 20 RgGuinier 2.3 nm Dmax 7.9 nm VolumePorod 27 nm3

SASDU99 – Apolipoprotein A-I dimer, C-terminally truncated Sample: Apolipoprotein A-I dimer, 43 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 0.1% sodium azide, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 20 RgGuinier 4.2 nm Dmax 14.0 nm VolumePorod 58 nm3

SASDUA9 – Apolipoprotein A-I monomer, C-terminally truncated, triply methionine-oxidised Sample: Apolipoprotein A-I monomer, 22 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 0.1% sodium azide, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 1 RgGuinier 2.5 nm Dmax 8.0 nm VolumePorod 32 nm3

SASDUB9 – Apolipoprotein A-I monomer, C-terminally truncated, SAXS construct G26R mutant Sample: Apolipoprotein A-I (G50R) monomer, 22 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 0.1% sodium azide, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Jun 27 RgGuinier 2.3 nm Dmax 7.9 nm VolumePorod 28 nm3

SASDUC9 – Full-length apolipoprotein A-I in complex with antigen-binding fragment 55201 Sample: Apolipoprotein A-I monomer, 28 kDa Homo sapiens protein Antigen-binding fragment 55201 monomer, 45 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 0.1% sodium azide, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 19 RgGuinier 4.6 nm Dmax 17.5 nm VolumePorod 99 nm3