Full-length SFPQ, and SFPQ1-598 homodimers

Submitted to SASBDB: 2024 Sep 18 Published in SASBDB: 2025 Jun 29

SASDV57 – Full-length SFPQ in high salt buffer Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Oct 22 RgGuinier 7.9 nm Dmax 42.5 nm VolumePorod 485 nm3

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein Buffer: 500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13 RgGuinier 7.6 nm Dmax 34.3 nm VolumePorod 400 nm3

SASDV77 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) in low salt buffer Sample: Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13 RgGuinier 6.1 nm Dmax 28.0 nm VolumePorod 315 nm3