Human FASTK preferentially binds single-stranded and G-rich RNA.

Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW
FEBS J (2025 Jul 1)

PMID: 40598781 doi: 10.1111/febs.70155

Submitted to SASBDB: 2024 Jun 20 Published in SASBDB: 2024 Jul 21

SASDVV2 – Fas-activated serine/threonine kinase/phosphoprotein FASTK (76-549) Sample: Fas-activated serine/threonine kinase (amino acids 76-549) monomer, 53 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 31 RgGuinier 3.6 nm Dmax 16.3 nm VolumePorod 96 nm3

SASDVW2 – Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549) Sample: Fas-activated serine/threonine kinase (amino acids 169-549) monomer, 43 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 31 RgGuinier 2.8 nm Dmax 12.0 nm VolumePorod 67 nm3