Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme.

Singhal A, Mrozowich T, Rivera C, Chaudhury SN, Xu L, Aguilar R, Badmalia M, Lee JT, Patel TR, Sanbonmatsu KY
Commun Biol (2026 Mar 23)

PMID: 41872304 doi: 10.1038/s42003-026-09819-0

Submitted to SASBDB: 2024 Jun 24 Published in SASBDB: 2026 Feb 4

SASDV63 – B2 SINE ribozyme wildtype Sample: B2 short interspaced nuclear element (SINE) RNA monomer, 57 kDa Mus musculus RNA Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 20 RgGuinier 5.4 nm Dmax 17.3 nm VolumePorod 131 nm3

SASDV93 – B2 SINE ribozyme with B2J Mutation Sample: B2 short interspaced nuclear element (SINE) RNA with two point mutations monomer, 57 kDa Mus musculus RNA Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 20 RgGuinier 5.5 nm Dmax 17.3 nm VolumePorod 136 nm3

SASDV83 – B2 SINE ribozyme with deletion mutation 96-105 Sample: B2 short interspaced nuclear element (SINE) RNA with deletion mutation 96-105 monomer, 54 kDa Mus musculus RNA Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Dec 18 RgGuinier 5.5 nm Dmax 17.5 nm VolumePorod 135 nm3

SASDV73 – B2 SINE ribozyme with deletion mutation 81-124 Sample: B2 short interspaced nuclear element (SINE) RNA with deletion mutation 81-124 dimer, 86 kDa Mus musculus RNA Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Aug 19 RgGuinier 8.1 nm Dmax 22.1 nm