Structural basis of microtubule-mediated signal transduction.

Choi SR, Blum TB, Giono M, Roy B, Vakonakis I, Schmid D, Oelgarth N, Ranganathan A, Gossert AD, Shivashankar GV, Zippelius A, Steinmetz MO
Cell (2025 Dec 8)

PMID: 41365297 doi: 10.1016/j.cell.2025.11.011

Submitted to SASBDB: 2024 Jun 18 Published in SASBDB: 2025 Dec 11

SASDV33 – N-terminal domain construct of hsGEFH1_190-582aa fit to DAMMIF and Alphafold2 models Sample: Isoform 1 of Rho guanine nucleotide exchange factor 2 monomer, 47 kDa Homo sapiens protein Buffer: 20mM Tris-Cl, 150mM NaCl, 1mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 19 RgGuinier 5.9 nm

SASDV43 – N-terminal domain construct of hsGEFH1_28-582aa fit to DAMMIF model and EOM Sample: Isoform 1 of Rho guanine nucleotide exchange factor 2 monomer, 64 kDa Homo sapiens protein Buffer: 20mM Tris-Cl, 150mM NaCl, 1mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 19 RgGuinier 3.7 nm

SASDV23 – N-terminal domain construct of hsGEFH1_28-582aa fit to various models Sample: Isoform 1 of Rho guanine nucleotide exchange factor 2 monomer, 64 kDa Homo sapiens protein Buffer: 20mM Tris-Cl, 150mM NaCl, 1mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 19 RgGuinier 3.7 nm