The pseudoknot structure of a viral RNA reveals a conserved mechanism for programmed exoribonuclease resistance

Sophie Korn Submitted to SASBDB: 2024 Jul 12
Published in SASBDB:

SASDVY4 – ST9a RNA, WT (59 nucleotides in 0 mM MgCl2; monomer)

ST9 WT (59 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (59 nt RNA) monomer, 19 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 26 nm3

SASDVX4 – ST9a RNA, WT (59 nucleotides in 10 mM MgCl2; monomer)

ST9 WT (59 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (59 nt RNA) monomer, 19 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
RgGuinier 1.9 nm
Dmax 6.7 nm
VolumePorod 24 nm3

SASDV25 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; monomer)

ST9 PKmutant (59 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 PKmutant (59 nt RNA) monomer, 19 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 29 nm3

SASDV35 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; dimer)

ST9 PKmutant (59 nt RNA) experimental SAS data
ST9 PKmutant (59 nt RNA) Kratky plot
Sample: ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
RgGuinier 3.8 nm
Dmax 12.5 nm
VolumePorod 73 nm3

SASDVZ4 – ST9a RNA, PKmutant (59 nucleotides in 10 mM MgCl2; dimer)

ST9 PKmutant (59 nt RNA) experimental SAS data
ST9 PKmutant (59 nt RNA) Kratky plot
Sample: ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30
RgGuinier 3.5 nm
Dmax 12.8 nm
VolumePorod 62 nm3

SASDVV4 – ST9a RNA, WT (54 nucleotides in 10 mM MgCl2; monomer)

ST9 WT (54 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (54 nt RNA) monomer, 17 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Mar 23
RgGuinier 1.9 nm
Dmax 5.8 nm
VolumePorod 21 nm3

SASDVW4 – ST9a RNA, WT (54 nucleotides in 1 mM MgCl2; monomer)

ST9 WT (54 nt RNA) experimental SAS data
PYMOL model
Sample: ST9 WT (54 nt RNA) monomer, 17 kDa RNA
Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH:
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Mar 23
RgGuinier 1.7 nm
Dmax 5.8 nm
VolumePorod 20 nm3