A conserved viral RNA fold enables nuclease resistance across kingdoms of life.

Gezelle JG, Korn SM, McDonald JT, Gong Z, Erickson A, Huang CH, Yang F, Cronin M, Kuo YW, Wimberly BT, Steckelberg AL
Nucleic Acids Res 53(16) (2025 Aug 27)

PMID: 40884403 doi: 10.1093/nar/gkaf840

Submitted to SASBDB: 2024 Jul 12 Published in SASBDB: 2025 Jul 12

SASDVY4 – ST9a RNA, WT (59 nucleotides in 0 mM MgCl2; monomer) Sample: ST9 WT (59 nt RNA) monomer, 19 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30 RgGuinier 2.1 nm Dmax 8.3 nm VolumePorod 26 nm3

SASDVX4 – ST9a RNA, WT (59 nucleotides in 10 mM MgCl2; monomer) Sample: ST9 WT (59 nt RNA) monomer, 19 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30 RgGuinier 1.9 nm Dmax 6.7 nm VolumePorod 24 nm3

SASDV25 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; monomer) Sample: ST9 PKmutant (59 nt RNA) monomer, 19 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30 RgGuinier 2.5 nm Dmax 8.8 nm VolumePorod 29 nm3

SASDV35 – ST9a RNA, PKmutant (59 nucleotides in 0 mM MgCl2; dimer) Sample: ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 0.0002% w/v NaN3, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30 RgGuinier 3.8 nm Dmax 12.5 nm VolumePorod 73 nm3

SASDVZ4 – ST9a RNA, PKmutant (59 nucleotides in 10 mM MgCl2; dimer) Sample: ST9 PKmutant (59 nt RNA) dimer, 38 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jan 30 RgGuinier 3.5 nm Dmax 12.8 nm VolumePorod 62 nm3

SASDVV4 – ST9a RNA, WT (54 nucleotides in 10 mM MgCl2; monomer) Sample: ST9 WT (54 nt RNA) monomer, 17 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 10 mM MgCl2, 0.0002% w/v NaN3, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Mar 23 RgGuinier 1.9 nm Dmax 5.8 nm VolumePorod 21 nm3

SASDVW4 – ST9a RNA, WT (54 nucleotides in 1 mM MgCl2; monomer) Sample: ST9 WT (54 nt RNA) monomer, 17 kDa RNA Buffer: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH: Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Mar 23 RgGuinier 1.7 nm Dmax 5.8 nm VolumePorod 20 nm3