The structural organisation of pentraxin-3 and its interactions with heavy chains of inter-α-inhibitor regulate crosslinking of the hyaluronan matrix

Shah A, Zhang X, Snee M, Lockhart-Cairns M, Levy C, Jowitt T, Birchenough H, Dean L, Collins R, Dodd R, Roberts A, Enghild J, Mantovani A, Fontana J, Baldock C, Inforzato A, Richter R, Day A
Matrix Biology 136:52-68 (2025 Apr)

doi: 10.1016/j.matbio.2025.01.002

Submitted to SASBDB: 2024 Aug 19 Published in SASBDB: 2025 Jul 27

SASDVA6 – Pentraxin-3 Sample: Pentraxin-related protein PTX3 octamer, 321 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 28 RgGuinier 8.6 nm Dmax 43.1 nm VolumePorod 658 nm3

SASDVB6 – Pentraxin-3 truncation mutant lacking the first 24 amino acids per mature promoter Sample: Pentraxin-related protein PTX3 (N-terminal deletion mutant) octamer, 301 kDa Homo sapiens protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 6 RgGuinier 8.4 nm Dmax 40.0 nm VolumePorod 647 nm3

SASDVC6 – Tetramer of Pentraxin-3 Sample: Pentraxin-related protein PTX3 (C317S, C318S) tetramer, 160 kDa Homo sapiens protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 6 RgGuinier 7.8 nm Dmax 26.0 nm VolumePorod 370 nm3