Conformational signatures induced by ubiquitin modification in the amyloid-forming tau repeat domain.

Viola G, Trivellato D, Laitaoja M, Jänis J, Felli IC, D'Onofrio M, Mollica L, Giachin G, Assfalg M
Proc Natl Acad Sci U S A 122(15):e2425831122 (2025 Apr 15)
PMID: 40198698
doi: 10.1073/pnas.2425831122 		Submitted to SASBDB: 2024 Aug 13
Published in SASBDB:

SASDV46 – Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD

Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) experimental SAS data
Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD Rg histogram
Sample: Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Buffer: 100 mM NaCl, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 33 nm3

SASDV56 – Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD synthetically linked to Ubiquitin at tau residue C317

Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A)Polyubiquitin-B experimental SAS data
Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD synthetically linked to Ubiquitin at tau residue C317 Rg histogram
Sample: Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
Buffer: 100 mM NaCl, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
RgGuinier 3.3 nm
Dmax 13.3 nm
VolumePorod 41 nm3

SASDV66 – Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD synthetically linked to Ubiquitin at tau residue C353

Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A)Polyubiquitin-B experimental SAS data
Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD synthetically linked to Ubiquitin at tau residue C353 Rg histogram
Sample: Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
Buffer: 100 mM NaCl, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
RgGuinier 3.1 nm
Dmax 12.1 nm
VolumePorod 33 nm3

SASDV76 – Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD synthetically linked to two Ubiquitins at tau residues C311 and C317

Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A)Polyubiquitin-BPolyubiquitin-B experimental SAS data
Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD synthetically linked to two Ubiquitins at tau residues C311 and C317 Rg histogram
Sample: Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) monomer, 14 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
Polyubiquitin-B monomer, 9 kDa Fukomys damarensis protein
Buffer: 100 mM NaCl, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
RgGuinier 3.3 nm
Dmax 12.2 nm
VolumePorod 59 nm3