Crystal structure of Isthmin-1 and reassessment of its functional role in pre-adipocyte signaling.

Li T, Stayrook SE, Li W, Wang Y, Li H, Zhang J, Liu Y, Klein DE
Nat Commun 16(1):3580 (2025 Apr 15)
PMID: 40234450
doi: 10.1038/s41467-025-58828-w 		Submitted to SASBDB: 2024 Sep 6
Published in SASBDB:

SASDVW6 – Monomeric Adhesion-associated domain in MUC4 and Other Proteins (AMOP-domain) of Isthmin-1

Isthmin-1 AMOP-domain experimental SAS data
Isthmin-1 AMOP-domain Kratky plot
Sample: Isthmin-1 AMOP-domain monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2023 Jun 21
RgGuinier 2.3 nm
Dmax 9.7 nm
VolumePorod 45264 nm3

SASDVX6 – Monomeric Adhesion-associated domain in MUC4 and Other Proteins (AMOP-domain) of Isthmin-1, I455E, F458E douple mutant

L-AMOP-IFEE experimental SAS data
L-AMOP-IFEE Kratky plot
Sample: L-AMOP-IFEE monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2023 Jun 21
RgGuinier 2.4 nm
Dmax 8.8 nm
VolumePorod 46874 nm3