Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA).

Yun MK, Subramanian C, Miller K, Jackson P, Radka CD, Rock CO
Biochemistry (2025 Jun 10)

PMID: 40494512 doi: 10.1021/acs.biochem.5c00168

Submitted to SASBDB: 2024 Sep 16 Published in SASBDB: 2025 Jun 12

SASDVC7 – Tetrameric L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli Sample: L-threonine dehydratase biosynthetic IlvA tetramer, 233 kDa Escherichia coli (strain … protein Buffer: 20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 14 RgGuinier 4.5 nm Dmax 15.6 nm VolumePorod 284 nm3

SASDVD7 – Regulatory domain of L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli Sample: L-threonine dehydratase biosynthetic IlvA Regulatory domain dimer, 46 kDa Escherichia coli (strain … protein Buffer: 20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 14 RgGuinier 2.3 nm Dmax 8.0 nm VolumePorod 50 nm3

SASDVE7 – Hexameric L-threonine dehydratase biosynthetic (IlvA) from Staphylococcus aureus Sample: L-threonine dehydratase biosynthetic IlvA hexamer, 288 kDa Staphylococcus aureus (strain … protein Buffer: 20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 14 RgGuinier 5.1 nm Dmax 17.1 nm VolumePorod 386 nm3