Probing the statistics of sequence-dependent DNA conformations in solution using SAXS

Heidar Koning

Submitted to SASBDB: 2024 Sep 18 Published in SASBDB: 2025 Aug 12

SASDT72 – GAGE6 60bp dsDNA oligonucleotide Sample: GAGE6 60bp dsDNA oligo monomer, 37 kDa Homo sapiens DNA Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 14 RgGuinier 4.7 nm Dmax 20.7 nm VolumePorod 101 nm3

SASDV87 – The 60bp dsDNA oligo GAGE6_1 Sample: GAGE6_1 dimer, 37 kDa Homo sapiens DNA Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Nov 28 RgGuinier 5.1 nm Dmax 19.2 nm VolumePorod 67 nm3

SASDV97 – The 60bp dsDNA oligo GAGE6_2 Sample: GAGE6_2 dimer, 37 kDa Homo sapiens DNA Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Nov 28 RgGuinier 5.2 nm Dmax 18.4 nm VolumePorod 72 nm3

SASDVA7 – The 60bp dsDNA oligo GAGE6_3 Sample: GAGE6_3 dimer, 37 kDa Homo sapiens DNA Buffer: 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Nov 28 RgGuinier 5.0 nm Dmax 21.4 nm VolumePorod 80 nm3