The mechanism behind acetyl-CoA-mediated activation in Mycobacterium tuberculosis isocitrate lyase 2 and the contribution of its unique protein features to allostery and selectivity

Ashish Sethi

Submitted to SASBDB: 2024 Sep 21 Published in SASBDB: 2026 Sep 21

SASDVM7 – Mycobacterium tuberculosis Isocitrate Lyase 2 Sample: Isocitrate lyase 2 monomer, 87 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Jun 27 RgGuinier 5.0 nm Dmax 19.5 nm VolumePorod 507 nm3

SASDVN7 – Mycobacterium tuberculosis Isocitrate Lyase 2 mutant (Q635C, V708C, V734C) Sample: Isocitrate lyase 2 (Q635C, V708C, V734C) monomer, 87 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Jun 27 RgGuinier 5.2 nm Dmax 20.6 nm VolumePorod 526 nm3

SASDVP7 – Mycobacterium tuberculosis Isocitrate Lyase 2 mutant (E640C, N731C, E733C) Sample: Isocitrate lyase 2 (E640C, N731C, E733C) monomer, 87 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Jun 27 RgGuinier 5.5 nm Dmax 23.9 nm VolumePorod 617 nm3