Biophysical characterization of honey bee virus untranslated regions

Jenna M Letain, Trushar R Patel
University of Lethbridge Dept. of Chemistry and Biochemistry Thesis (2024)

URL: https://hdl.handle.net/10133/6969 		Submitted to SASBDB: 2024 Oct 8
Published in SASBDB:

SASDVW7 – Sacbrood virus 3' untranslated region RNA (SBV 3'UTR)

SBV 3'UTR experimental SAS data
DAMMIN model
Sample: SBV 3'UTR monomer, 31 kDa Sacbrood virus RNA
Buffer: 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, 15 mM KCl, 5% glycerol, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Apr 28
RgGuinier 3.6 nm
Dmax 10.8 nm
VolumePorod 52 nm3

SASDVX7 – Sacbrood virus 5' untranslated region RNA (SBV 5'UTR)

SBV 5'UTR experimental SAS data
DAMMIN model
Sample: SBV 5'UTR monomer, 61 kDa Sacbrood virus RNA
Buffer: 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, 15 mM KCl, 5% glycerol, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 19
RgGuinier 4.9 nm
Dmax 15.1 nm
VolumePorod 120 nm3