Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody.

Semwal S, Karamolegkou M, Flament S, Raouraoua N, Verstraete K, Thureau A, Wien F, Bray F, Savvides SN, Bouckaert J
Acta Crystallogr F Struct Biol Commun (2025 Jan 1)

PMID: 39718025 doi: 10.1107/S2053230X24012159

Submitted to SASBDB: 2024 Nov 25 Published in SASBDB: 2024 Dec 30

SASDWK2 – Mannitou IgM Fab Monomer Sample: IgM Mannitou Fab Heavy Chain monomer, 26 kDa Mus musculus protein IgM Mannitou Fab Light Chain monomer, 24 kDa Mus musculus protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Jun 5 RgGuinier 2.8 nm Dmax 12.5 nm VolumePorod 74 nm3