A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.

Nguyen BV, Özden C, Dong K, Koc OC, Torres-Ocampo AP, Dziedzic N, Flaherty D, Huang J, Sankara S, Abromson NL, Tomchick DR, Fissore RA, Chen J, Garman SC, Stratton MM
Nat Commun 16(1):8461 (2025 Sep 26)
PMID: 41006217
doi: 10.1038/s41467-025-63249-w 		Submitted to SASBDB: 2024 Oct 5
Published in SASBDB:

SASDVU7 – Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 14 as the linker region

Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344) experimental SAS data
GASBOR model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344) dodecamer, 620 kDa Homo sapiens protein
Buffer: 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol, pH:
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Apr 17
RgGuinier 6.8 nm
Dmax 25.7 nm
VolumePorod 1437 nm3

SASDVV7 – Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 18 as the linker region

Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) experimental SAS data
GASBOR model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein
Buffer: 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol, pH:
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Apr 17
RgGuinier 6.0 nm
Dmax 19.0 nm
VolumePorod 1080 nm3