Structural and Functional Investigation of Putative Peptidase from Mycolicibacterium phlei : An Exclusive Endopeptidase among S9C Subfamily

Chandravanshi K, Jamdar S, Singh R, Kumar A, Mahato A, Agrawal R, Kumar S, Kumar A, Makde R
Biochemistry (2025 May 28)

doi: 10.1021/acs.biochem.5c00047 		Submitted to SASBDB: 2024 Oct 14
Published in SASBDB:

SASDVF8 – S9 peptidase form Mycobacterium phlei (8 mg/ml)

Peptidase S9 experimental SAS data
GASBOR model
Sample: Peptidase S9 tetramer, 304 kDa Mycolicibacterium phlei DSM … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 21
RgGuinier 5.2 nm
Dmax 15.8 nm
VolumePorod 559 nm3

SASDVG8 – S9 peptidase form Mycobacterium phlei (6 mg/ml)

Peptidase S9 experimental SAS data
GASBOR model
Sample: Peptidase S9 tetramer, 304 kDa Mycolicibacterium phlei DSM … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 21
RgGuinier 5.0 nm
Dmax 15.7 nm
VolumePorod 546 nm3

SASDVH8 – S9 peptidase form Mycobacterium phlei (4 mg/ml)

Peptidase S9 experimental SAS data
GASBOR model
Sample: Peptidase S9 tetramer, 304 kDa Mycolicibacterium phlei DSM … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 21
RgGuinier 5.1 nm
Dmax 16.2 nm
VolumePorod 563 nm3