Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation

Heidar Koning Submitted to SASBDB: 2024 Nov 4
Published in SASBDB:

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Splicing factor, proline- and glutamine-rich experimental SAS data
Splicing factor, proline- and glutamine-rich Kratky plot
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer: 150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20
RgGuinier 6.1 nm
Dmax 22.0 nm
VolumePorod 181 nm3