Cryo-EM led analysis of open and closed conformations of Chagas vaccine candidate TcPOP.

Batra S, Olmo F, Ragan TJ, Kaplan M, Calvaresi V, Frank AM, Lancey C, Assadipapari M, Ying C, Struwe WB, Hesketh EL, Kelly JM, Barfod L, Campeotto I
Nat Commun 16(1):7164 (2025 Aug 5)
PMID: 40764299
doi: 10.1038/s41467-025-62068-3 		Submitted to SASBDB: 2024 Dec 7
Published in SASBDB:

SASDW23 – SEC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)

Prolyl endopeptidase experimental SAS data
ALPHAFOLD model
Sample: Prolyl endopeptidase monomer, 78 kDa Trypanosoma cruzi protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Apr 24
RgGuinier 3.0 nm
Dmax 0.0 nm
VolumePorod 142 nm3

SASDW33 – STATIC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)

Prolyl endopeptidase experimental SAS data
Prolyl endopeptidase Kratky plot
Sample: Prolyl endopeptidase monomer, 78 kDa Trypanosoma cruzi protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Apr 24
RgGuinier 3.8 nm
Dmax 0.0 nm
VolumePorod 170 nm3