Structure of the Thomasclavelia ramosa immunoglobulin A protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases.

Tran N, Frenette A, Holyoak T
Proc Natl Acad Sci U S A 122(35):e2503549122 (2025 Sep 2)

PMID: 40854123 doi: 10.1073/pnas.2503549122

Submitted to SASBDB: 2024 Dec 6 Published in SASBDB: 2025 Aug 11

SASDWH3 – Thomasclavelia ramosa immunoglobulin A protease N-terminal domain Sample: IgA protease monomer, 32 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 1.9 nm Dmax 6.0 nm VolumePorod 38 nm3

SASDWJ3 – Thomasclavelia ramosa immunoglobulin A protease middle (protease) domain Sample: IgA protease monomer, 55 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 2.6 nm Dmax 8.8 nm VolumePorod 82 nm3

SASDWK3 – Thomasclavelia ramosa immunoglobulin A protease middle domain with C-terminal domain #1 Sample: IgA protease monomer, 63 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 2.7 nm Dmax 9.2 nm VolumePorod 89 nm3

SASDWL3 – Thomasclavelia ramosa immunoglobulin A protease C-terminal domain #2 Sample: IgA protease monomer, 14 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 2.5 nm Dmax 9.6 nm VolumePorod 12 nm3

SASDWM3 – Thomasclavelia ramosa immunoglobulin A protease N-terminal domain with middle (protease) domain Sample: IgA protease monomer, 88 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 3.3 nm Dmax 10.8 nm VolumePorod 108 nm3

SASDWN3 – Thomasclavelia ramosa immunoglobulin A protease C-terminal tail Sample: IgA protease monomer, 39 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 5.3 nm Dmax 21.5 nm VolumePorod 52 nm3

SASDWP3 – Thomasclavelia ramosa immunoglobulin A protease middle (protease) domain with C-terminal tail Sample: IgA protease monomer, 95 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 5.7 nm Dmax 24.0 nm VolumePorod 160 nm3

SASDWQ3 – Thomasclavelia ramosa full-length immunoglobulin A protease Sample: IgA protease monomer, 131 kDa Thomasclavelia ramosa protein Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26 RgGuinier 5.5 nm Dmax 24.5 nm VolumePorod 188 nm3