Nanobodies as tools for studying human frataxin biology.

Pignataro MF, Fernández NB, Garay-Alvarez A, Pavan MF, Molina R, Muñoz IG, Grossi J, Noguera M, Vila A, García AE, Gentili HG, Rodríguez NA, Aran M, Parreño V, Bok M, Hermoso JA, Ibañez LI, Santos J
Commun Biol (2026 Jan 3)
PMID: 41484353
doi: 10.1038/s42003-025-09458-x 		Submitted to SASBDB: 2025 Jan 27
Published in SASBDB:

SASDWT6 – Frataxin bound to tailored nanobody 16C10

Frataxin, mitochondrialNanobody 16C10 experimental SAS data
COOT model
Sample: Frataxin, mitochondrial monomer, 14 kDa Homo sapiens protein
Nanobody 16C10 monomer, 15 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Sep 18
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 37 nm3

SASDWS6 – Frataxin bound to tailored nanobody 28F6

Frataxin, mitochondrialNanobody 28F6 experimental SAS data
ALPHAFOLD model
Sample: Frataxin, mitochondrial monomer, 14 kDa Homo sapiens protein
Nanobody 28F6 monomer, 15 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Sep 18
RgGuinier 2.2 nm
Dmax 7.4 nm
VolumePorod 36 nm3