Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex.

Stafflinger H, Neißner K, Bartsch S, Pichler AK, Bartosik K, Dhamotharan K, Abele R, Duchardt-Ferner E, Micura R, Schindelin H, Wöhnert J
Nucleic Acids Res 53(22) (2025 Nov 26)
PMID: 41422825
doi: 10.1093/nar/gkaf1315 		Submitted to SASBDB: 2025 Jan 13
Published in SASBDB:

SASDWH4 – Class V GTP aptamer

Class V GTP aptamer apo experimental SAS data
Class V GTP aptamer apo Kratky plot
Sample: Class V GTP aptamer apo, 22 kDa Escherichia coli RNA
Buffer: 50 mM Bis-Tris, 50 mM KCl, 2 mM MgCl2, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Jul 19
RgGuinier 3.3 nm
Dmax 15.3 nm
VolumePorod 99 nm3

SASDWJ4 – Class V GTP aptamer complex with GTP

Class V GTP aptamerGuanosine triphosphate experimental SAS data
COOT model
Sample: Class V GTP aptamer monomer, 22 kDa Escherichia coli RNA
Guanosine triphosphate monomer, 1 kDa
Buffer: 50 mM Bis-Tris, 50 mM KCl, 2 mM MgCl2, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Jul 19
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 34 nm3