Structural insights into the nature of static and dynamic apoferritin dimers

Aleksei Tsarenko

Submitted to SASBDB: 2025 Jan 28 Published in SASBDB: 2026 Jan 28

SASDWV6 – SEC-SAXS data for the monomeric (24mer) fraction of horse spleen apoferritin Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 0.9 mM CaCl2, 0.5 mM MgCl2, pH: 7.4 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 May 24 RgGuinier 5.4 nm Dmax 13.5 nm VolumePorod 651 nm3

SASDWW6 – SEC-SAXS data for the total dimer (2 x 24mer) fraction of horse spleen apoferritin Sample: Ferritin light chain, 953 kDa Equus caballus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 0.9 mM CaCl2, 0.5 mM MgCl2, pH: 7.4 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 May 24 RgGuinier 7.7 nm Dmax 25.0 nm VolumePorod 1751 nm3

SASDWX6 – SEC-SAXS data for presumably dynamic dimers (2 x 24mer) of horse spleen apoferritin Sample: Ferritin light chain, 953 kDa Equus caballus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 0.9 mM CaCl2, 0.5 mM MgCl2, pH: 7.4 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 May 24 RgGuinier 8.0 nm Dmax 26.8 nm VolumePorod 1369 nm3

SASDWY6 – SEC-SAXS data for presumably disulfide-bounded dimers (2 x 24mer) of horse spleen apoferritin Sample: Ferritin light chain, 953 kDa Equus caballus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 0.9 mM CaCl2, 0.5 mM MgCl2, pH: 7.4 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 May 24 RgGuinier 8.1 nm Dmax 25.0 nm VolumePorod 1677 nm3