Unique conformational dynamics and protein recognition of A-to-I hyper-edited dsRNA.

Müller-Hermes C, Piomponi V, Hilber S, Asami S, Kreutz C, Bussi G, Sattler M
Nucleic Acids Res 53(12) (2025 Jun 20)
PMID: 40568935
doi: 10.1093/nar/gkaf550 		Submitted to SASBDB: 2025 Jan 22
Published in SASBDB:

SASDWL5 – I-RNA, an A-to-I hyper-edited 20mer dsRNA

I-RNA, Top-strandI-RNA, Bottom-strand experimental SAS data
I-RNA, Top-strand I-RNA, Bottom-strand Kratky plot
Sample: I-RNA, Top-strand monomer, 7 kDa RNA
I-RNA, Bottom-strand monomer, 7 kDa RNA
Buffer: 25 mM sodium phosphate 25 mM sodium chloride, pH: 6.4
Experiment: SAXS data collected at BM29, ESRF on 2022 Nov 9
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 17 nm3

SASDWM5 – A-RNA, non-edited 20mer dsRNA

A-RNA, Top-StrandA-RNA, Bottom-Strand experimental SAS data
A-RNA, Top-Strand A-RNA, Bottom-Strand Kratky plot
Sample: A-RNA, Top-Strand monomer, 6 kDa RNA
A-RNA, Bottom-Strand monomer, 6 kDa RNA
Buffer: 25 mM sodium phosphate 25 mM sodium chloride, pH: 6.4
Experiment: SAXS data collected at BM29, ESRF on 2022 Nov 9
RgGuinier 1.8 nm
Dmax 6.2 nm
VolumePorod 17 nm3